Circle the best option that explains how are the dideoxynucleotides used in next-generation sequencing different from those used in original Sanger sequencing method.
A. They lack 2'OH group
B. They have 2'OH and 3'OH group
C. The H attached to 3 ′ C in ddNTP can be chemically reverted to 3'OH group, so chain termination can be reversed
D. They lack 3'OH group