mRNA treatment. Eukaryotic pre-mRNAs undergo extensive processing before they are ready for translation. Eukaryotic protein-coding sequences are not as continuous as prokaryotes. Code sequences (exons) are interrupted by non-code introns. Non-coded introns need to be removed in order to obtain translatable mRNA.
How are mRNAs processed in eukaryotic cells?
In the cell nucleus, pre-mRNA is produced by transcription of the DNA region of the linear chromosome. This transcript needs to be processed (splicing and adding a 5'cap and poly-A tail) while still in the nucleus to become a mature mRNA.
mRNA is produced during the transcription process in which an enzyme (RNA polymerase) converts a gene into primary transcript mRNA (also known as pre-mRNA). This pre-mRNA usually contains introns, which are regions that do not encode the final amino acid sequence.
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